sTLR2 RAPID (10 tests)

Immunochromatographic assay for the semi-quantitative measurement of human soluble Toll-like receptor 2 in serum. Each kit contains sufficient material to test 10 samples.

• 20µL sample (plasma, serum)
• Results in 10 minutes
  
• UKCA certified

Product code: STLR2R

Contents

• 10 foil pouches containing one test device
• sTLR2 diluent bottle

Store refrigerated.

Background

A soluble form of Toll-like receptor 2 (sTLR2) is present in human serum, plasma and other bodily fluids and showed anti-inflammatory potential (1). It is released early upon infection/cell activation and acts as a decoy receptor to limit inflammation. Elevated levels of sTLR2 may be present early in patients with sepsis, and other infective and non-infective inflammatory conditions.

How the test works

The test is performed by adding serum to the sample well of the sTLR2 Rapid device, followed by sTLR2 Rapid diluent. Any sTLR2 in the sample first binds to a specific mouse anti-TLR2 monoclonal antibody which has been conjugated to a gold nanoparticle. The nanoparticle-antibody-sTLR2 complex then flows laterally through a membrane where a second specific mouse anti-TLR2 monoclonal antibody is permanently adsorbed to the membrane opposite the letter ‘T’ on the test device. An anti-mouse antibody is similarly permanently adsorbed to the membrane opposite the letter ‘C’ on the test device, which serves as a control. The result is read after 10 minutes.

The colour intensity of the line formed opposite the letter ‘T’ on the test device is proportional to the amount of human sTLR2 in the sample. A semi-quantitative estimate of the level of human sTLR2 is made with reference to the intensity of the test line.

The rapid test is calibrated against a human serum pool measured by ELISA, with reference to recombinant human TLR2.

For professional use only.

UK Patent Application GB2502531.3.